forkhead box protein m1  (Proteintech)

Journal: Scientific Reports

Article Title: The beneficial effects of a muscarinic agonist on pancreatic β-cells

doi: 10.1038/s41598-019-52691-8

Figure Lengend Snippet: The muscarinic agonist increases the phosphorylation of Akt and expression of cyclin D2 protein in mouse islets. ( A ) Phosphorylation of Akt and ( B ) expression of cyclin D2 protein were evaluated in isolated islets from wild-type (WT) mice and insulin-receptor substrate 2 ( IRS-2 ) −/− mice orally administered bethanechol (Bch; 1 µg/g body weight) once a day for 1 week (n = 4–9). ( C ) Protein levels of FoxO1, FoxM1 and PDX1 were analysed in WT mice administered Bch (1 µg/g) once a day for 1 week (n = 3–7). The data represent the mean ± standard error of the mean (SEM). * P < 0.05 vs. control.

Article Snippet: The lysed islets were subjected to centrifugation and the extracts were subjected to immunoblotting with antibodies to the following proteins: Akt, phospho-Akt, forkhead transcription factor (FoxO1) (Cell Signalling Technology); cyclin D2, forkhead box protein M1 (FoxM1) (Santa Cruz); pancreatic and duodenal homeobox 1 (PDX1) (Merck); or β actin (Abcam).

Techniques: Phospho-proteomics, Expressing, Isolation, Control